MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : STM_v1_0 [2].
Target metabolite : 12dgr120_p
List of minimal gene deletion strategies (Download)

Gene deletion strategy (22 of 110: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 29
  Gene deletion: STM3646 STM2285 STM3526 STM0169 STM0861 STM4326 STM0321 STM2947 STM3709 STM1135 STM0369 STM1448 STM1326 STM4062 STM3069 STM4184 STM4484 STM2317 STM3179 STM1480 STM4126 STM3802 STM2196 STM3240 STM0007 STM2473 STM3708 STM2971 STM1826   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.215733 (mmol/gDw/h)
  Minimum Production Rate : 0.085707 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 18.500000
  EX_glc__D_e : 5.000000
  EX_nh4_e : 2.377405
  EX_pi_e : 0.191317
  EX_k_e : 0.038314
  EX_so4_e : 0.026316
  EX_mg2_e : 0.001703
  EX_fe2_e : 0.001581
  EX_ca2_e : 0.001022
  EX_cl_e : 0.001022
  EX_cu2_e : 0.000681
  EX_mn2_e : 0.000681
  EX_mobd_e : 0.000681
  EX_zn2_e : 0.000681
  EX_cobalt2_e : 0.000681

Product: (mmol/gDw/h)
  EX_h2o_e : 25.373879
  EX_co2_e : 19.340784
  EX_h_e : 1.828901
  Auxiliary production reaction : 0.085707
  DM_hmfurn_c : 0.000096

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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