MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iLB1027_lipid [2].
Target metabolite : cl_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (22 of 23: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 32
  Gene deletion: PHATRDRAFT_20342 PHATRDRAFT_800 PHATRDRAFT_42015 PHATRDRAFT_55192 Phatr3_EG02232 PHATRDRAFT_55126 PHATRDRAFT_48983 PHATRDRAFT_22404 PHATRDRAFT_3046 PHATRDRAFT_34120 PHATRDRAFT_45017 PHATRDRAFT_14762 PHATRDRAFT_38509 PHATRDRAFT_31906 PHATRDRAFT_28191 Phatr3_EG02361 PHATRDRAFT_47090 PHATRDRAFT_33720 PHATRDRAFT_52268 PHATRDRAFT_859 PHATRDRAFT_46400 PHATRDRAFT_12431 PHATRDRAFT_44546 PHATRDRAFT_11273 PHATRDRAFT_22117 PHATRDRAFT_15777 PHATRDRAFT_43697 PHATRDRAFT_45428 PHATRDRAFT_28181 PHATRDRAFT_12452 Phatr3_EG02611 PHATRDRAFT_23365   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.118159 (mmol/gDw/h)
  Minimum Production Rate : 0.202826 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_photon_e : 1000.000000
  EX_co2_e : 94.983688
  EX_h2o_e : 87.760527
  EX_cl_e : 51.993507
  EX_no3_e : 1.760000
  EX_pi_e : 0.029340
  EX_so4_e : 0.021013
  EX_mg2_e : 0.002112

Product: (mmol/gDw/h)
  EX_o2_e : 79.673121
  EX_h_e : 52.793974
  Auxiliary production reaction : 51.993507
  SK_for_c : 42.050970
  EX_lac__D_e : 12.556875
  DM_indole_c : 1.181702
  DM_biomass_c : 0.118159
  EX_etoh_e : 0.097874

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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