MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iLB1027_lipid [2].
Target metabolite : cys__L_m
List of minimal gene deletion strategies (Download)

Gene deletion strategy (10 of 25: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 13
  Gene deletion: PHATRDRAFT_10257 PHATRDRAFT_draft870 PHATRDRAFT_10073 PHATRDRAFT_30145 PHATRDRAFT_12762 PHATRDRAFT_3046 PHATRDRAFT_19708 PHATRDRAFT_12331 PHATRDRAFT_11414 PHATRDRAFT_32849 PHATRDRAFT_draft1517 PHATRDRAFT_28585 PHATRDRAFT_43697   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.275268 (mmol/gDw/h)
  Minimum Production Rate : 0.412774 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_photon_e : 1000.000000
  EX_h2o_e : 108.233458
  EX_co2_e : 88.651923
  EX_no3_e : 1.760000
  EX_so4_e : 0.461727
  EX_pi_e : 0.068351
  EX_mg2_e : 0.004919

Product: (mmol/gDw/h)
  EX_o2_e : 104.324488
  SK_for_c : 27.767993
  EX_h_e : 25.056946
  EX_etoh_e : 23.284984
  Auxiliary production reaction : 0.412774
  DM_biomass_c : 0.275268

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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