MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iLJ478 [2].
Target metabolite : leu__L_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (6 of 14: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 5
  Gene deletion: TM1385 TM1640 TM1217 TM0064 TM0296   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.077551 (mmol/gDw/h)
  Minimum Production Rate : 2.173621 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_s_e : 4.000000
  EX_h2o_e : 3.472676
  EX_nh4_e : 2.977913
  EX_malt_e : 2.794000
  EX_pi_e : 0.059619
  SK_apoACP_c : 0.000217

Product: (mmol/gDw/h)
  EX_h2_e : 11.288901
  EX_co2_e : 10.862723
  EX_h_e : 6.336872
  EX_h2s_e : 3.981166
  EX_ac_e : 3.433270
  Auxiliary production reaction : 2.173621
  SK_gcald_c : 0.003878
  SK_5mta_c : 0.000543
  SK_pap_c : 0.000217

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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