MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : 12ppd__S_e
List of minimal gene deletion strategies (Download)

Gene deletion strategy (69 of 92: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 33
  Gene deletion: b3553 b1478 b3942 b1732 b1241 b0351 b3844 b1004 b3713 b1109 b0046 b2463 b2210 b1033 b3551 b3945 b1602 b0153 b4219 b1832 b1778 b0584 b0755 b3612 b0529 b1380 b1710 b2480 b1695 b0606 b2285 b1010 b3924   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.506986 (mmol/gDw/h)
  Minimum Production Rate : 0.575647 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 35.806042
  EX_glc__D_e : 10.000000
  EX_nh4_e : 5.475407
  EX_pi_e : 0.489042
  EX_so4_e : 0.127669
  EX_k_e : 0.098960
  EX_fe2_e : 0.008143
  EX_mg2_e : 0.004398
  EX_cl_e : 0.002639
  EX_ca2_e : 0.002639
  EX_cu2_e : 0.000359
  EX_mn2_e : 0.000350
  EX_zn2_e : 0.000173
  EX_ni2_e : 0.000164
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 50.276062
  EX_co2_e : 37.463608
  EX_h_e : 4.658391
  EX_12ppd__S_e : 0.575647
  DM_5drib_c : 0.000114
  DM_4crsol_c : 0.000113

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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