MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : 14dhncoa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (58 of 84: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 43
  Gene deletion: b3553 b4382 b0238 b0125 b4384 b2744 b3708 b3008 b3752 b0871 b2883 b0120 b1982 b0477 b2797 b3117 b1814 b4471 b1033 b2440 b0261 b0507 b3709 b2406 b3161 b0112 b2868 b4064 b4464 b0114 b0886 b2366 b2492 b0904 b3035 b2578 b1533 b3927 b1473 b0494 b4141 b1798 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.751526 (mmol/gDw/h)
  Minimum Production Rate : 0.103501 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 24.130042
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.845133
  EX_pi_e : 1.035430
  EX_so4_e : 0.292750
  EX_k_e : 0.146693
  EX_fe2_e : 0.012070
  EX_mg2_e : 0.006519
  EX_ca2_e : 0.003912
  EX_cl_e : 0.003912
  EX_cu2_e : 0.000533
  EX_mn2_e : 0.000519
  EX_zn2_e : 0.000256
  EX_ni2_e : 0.000243
  EX_cobalt2_e : 0.000019

Product: (mmol/gDw/h)
  EX_h2o_e : 48.484052
  EX_co2_e : 25.835381
  EX_h_e : 7.220032
  Auxiliary production reaction : 0.103501
  EX_ade_e : 0.000841
  DM_5drib_c : 0.000504
  DM_4crsol_c : 0.000168

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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