MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : 4ppcys_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (69 of 73: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 34
  Gene deletion: b4467 b2836 b2242 b0474 b2518 b3831 b2781 b0030 b1004 b3713 b1109 b0046 b1612 b1611 b4122 b0651 b2162 b1759 b2210 b4161 b1415 b4138 b4123 b0621 b4381 b2406 b2197 b3028 b3918 b1912 b1206 b2285 b3893 b1474   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.532081 (mmol/gDw/h)
  Minimum Production Rate : 0.250256 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 992.955861
  EX_o2_e : 280.062886
  EX_glc__D_e : 10.000000
  EX_nh4_e : 7.063019
  EX_pi_e : 0.794221
  EX_so4_e : 0.414961
  EX_k_e : 0.103858
  EX_mg2_e : 0.004616
  EX_ca2_e : 0.002769
  EX_cl_e : 0.002769
  EX_cu2_e : 0.000377
  EX_mn2_e : 0.000368
  EX_zn2_e : 0.000181
  EX_ni2_e : 0.000172
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_fe3_e : 999.991454
  EX_h2o_e : 549.232674
  EX_co2_e : 31.058863
  EX_succ_e : 0.554849
  EX_ura_e : 0.377324
  Auxiliary production reaction : 0.280973
  EX_for_e : 0.001305
  DM_5drib_c : 0.000120
  DM_4crsol_c : 0.000119

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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