MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : 5aizc_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (20 of 80: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 25
  Gene deletion: b4382 b4269 b0493 b3588 b3003 b3011 b1241 b0351 b4384 b0871 b2779 b3617 b0030 b2407 b1982 b3665 b0261 b0507 b2406 b0112 b0114 b0529 b2492 b0904 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.471868 (mmol/gDw/h)
  Minimum Production Rate : 1.051529 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 23.649377
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.459369
  EX_pi_e : 1.506696
  EX_so4_e : 0.118826
  EX_k_e : 0.092105
  EX_fe2_e : 0.007579
  EX_mg2_e : 0.004093
  EX_cl_e : 0.002456
  EX_ca2_e : 0.002456
  EX_cu2_e : 0.000335
  EX_mn2_e : 0.000326
  EX_zn2_e : 0.000161
  EX_ni2_e : 0.000152
  EX_cobalt2_e : 0.000012

Product: (mmol/gDw/h)
  EX_h2o_e : 47.178653
  EX_co2_e : 23.164792
  EX_h_e : 9.991992
  EX_acald_e : 1.708140
  Auxiliary production reaction : 1.051529
  EX_ade_e : 0.241729
  DM_5drib_c : 0.241518
  EX_glyclt_e : 0.241517
  DM_4crsol_c : 0.241306

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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