MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : 6hmhpt_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (32 of 82: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 25
  Gene deletion: b4384 b3708 b3008 b0871 b0030 b2407 b1982 b2797 b3117 b1814 b4471 b4381 b2406 b0452 b0114 b0886 b2366 b2492 b0904 b2578 b1533 b3927 b2835 b0516 b1518   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.780963 (mmol/gDw/h)
  Minimum Production Rate : 0.179661 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 25.467707
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.334742
  EX_pi_e : 0.753322
  EX_so4_e : 0.196662
  EX_k_e : 0.152439
  EX_fe2_e : 0.012543
  EX_mg2_e : 0.006775
  EX_ca2_e : 0.004065
  EX_cl_e : 0.004065
  EX_cu2_e : 0.000554
  EX_mn2_e : 0.000540
  EX_zn2_e : 0.000266
  EX_ni2_e : 0.000252
  EX_cobalt2_e : 0.000020

Product: (mmol/gDw/h)
  EX_h2o_e : 49.106936
  EX_co2_e : 26.683571
  EX_h_e : 8.076207
  Auxiliary production reaction : 0.179661
  EX_alltn_e : 0.000526
  DM_mththf_c : 0.000350
  DM_5drib_c : 0.000176
  DM_4crsol_c : 0.000174

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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