MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : 6hmhpt_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (57 of 82: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 29
  Gene deletion: b3553 b3399 b1241 b0351 b4069 b2502 b2744 b3115 b1849 b2296 b2779 b2926 b1982 b3616 b3589 b1033 b0675 b2361 b0261 b4388 b4381 b2406 b0112 b2789 b3127 b0114 b0529 b2492 b0904   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.407460 (mmol/gDw/h)
  Minimum Production Rate : 1.540451 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 27.584996
  EX_nh4_e : 12.113910
  EX_glc__D_e : 10.000000
  EX_pi_e : 0.393039
  EX_so4_e : 0.102607
  EX_k_e : 0.079533
  EX_fe2_e : 0.006544
  EX_mg2_e : 0.003535
  EX_cl_e : 0.002121
  EX_ca2_e : 0.002121
  EX_cu2_e : 0.000289
  EX_mn2_e : 0.000282
  EX_zn2_e : 0.000139
  EX_ni2_e : 0.000132
  EX_cobalt2_e : 0.000010

Product: (mmol/gDw/h)
  EX_h2o_e : 50.003098
  EX_co2_e : 23.816450
  EX_h_e : 15.794834
  EX_ac_e : 2.785712
  EX_glyclt_e : 1.540724
  Auxiliary production reaction : 1.540451
  DM_oxam_c : 0.011118
  DM_5drib_c : 0.000273
  DM_4crsol_c : 0.000091

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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