MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : adpglc_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (32 of 73: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 26
  Gene deletion: b3399 b4382 b1241 b0351 b4069 b4384 b2744 b3115 b1849 b2296 b2407 b3616 b3589 b1623 b3665 b0261 b0411 b0112 b3654 b3714 b3664 b0114 b0529 b2492 b0904 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.527030 (mmol/gDw/h)
  Minimum Production Rate : 0.519292 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 20.283639
  EX_nh4_e : 10.253688
  EX_glc__D_e : 10.000000
  EX_pi_e : 1.546960
  EX_so4_e : 0.132717
  EX_k_e : 0.102873
  EX_fe2_e : 0.008465
  EX_mg2_e : 0.004572
  EX_ca2_e : 0.002743
  EX_cl_e : 0.002743
  EX_cu2_e : 0.000374
  EX_mn2_e : 0.000364
  EX_zn2_e : 0.000180
  EX_ni2_e : 0.000170
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 46.702304
  EX_co2_e : 19.618765
  EX_h_e : 8.672616
  EX_thymd_e : 0.982674
  Auxiliary production reaction : 0.519292
  EX_ac_e : 0.306830
  DM_5drib_c : 0.000119
  DM_4crsol_c : 0.000118

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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