MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : ahcys_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (80 of 80: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 61
  Gene deletion: b4382 b4269 b0493 b3588 b3003 b3011 b1241 b0351 b4384 b3708 b3008 b1479 b0910 b3752 b0512 b0871 b2407 b3124 b3236 b2687 b3962 b1982 b0104 b2797 b3117 b1814 b4471 b3616 b3589 b4267 b0675 b1748 b1014 b0822 b2913 b2406 b0112 b2789 b3127 b0511 b4265 b2975 b0114 b3603 b0755 b3612 b0306 b1539 b3605 b2492 b0904 b1533 b3825 b1380 b0325 b0789 b1249 b0508 b4266 b3662 b0221   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.408720 (mmol/gDw/h)
  Minimum Production Rate : 0.008380 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 35.634653
  EX_glc__D_e : 10.000000
  EX_nh4_e : 4.945763
  EX_so4_e : 0.591555
  EX_pi_e : 0.394253
  EX_k_e : 0.079779
  EX_fe2_e : 0.006564
  EX_mg2_e : 0.003546
  EX_ca2_e : 0.002127
  EX_cl_e : 0.002127
  EX_cu2_e : 0.000290
  EX_mn2_e : 0.000282
  EX_zn2_e : 0.000139
  EX_ni2_e : 0.000132
  EX_cobalt2_e : 0.000010

Product: (mmol/gDw/h)
  EX_h2o_e : 50.176119
  EX_co2_e : 38.302678
  EX_h_e : 3.309842
  EX_acald_e : 1.200201
  EX_met__L_e : 0.480252
  Auxiliary production reaction : 0.008380
  DM_5drib_c : 0.000274
  EX_xan_e : 0.000274
  DM_4crsol_c : 0.000091

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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