MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : ahdt_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (67 of 83: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 44
  Gene deletion: b3553 b3399 b4069 b4384 b2744 b3752 b2297 b2458 b3844 b1004 b3713 b1109 b0046 b3236 b2883 b1638 b1779 b0937 b1982 b4139 b1033 b1623 b4014 b0261 b2976 b0411 b2799 b3945 b1602 b0507 b4381 b1727 b0114 b0529 b2492 b0904 b2954 b3927 b3029 b1380 b2660 b3662 b2285 b1007   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.412816 (mmol/gDw/h)
  Minimum Production Rate : 0.479271 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 994.520255
  EX_o2_e : 287.414060
  EX_glc__D_e : 10.000000
  EX_nh4_e : 6.856580
  EX_pi_e : 1.836017
  EX_so4_e : 0.103955
  EX_k_e : 0.080579
  EX_mg2_e : 0.003581
  EX_ca2_e : 0.002149
  EX_cl_e : 0.002149
  EX_cu2_e : 0.000293
  EX_mn2_e : 0.000285
  EX_zn2_e : 0.000141
  EX_ni2_e : 0.000133
  EX_cobalt2_e : 0.000010

Product: (mmol/gDw/h)
  EX_fe3_e : 999.993370
  EX_h2o_e : 554.230130
  EX_co2_e : 37.484443
  Auxiliary production reaction : 0.479271
  EX_etoh_e : 0.387006
  EX_ac_e : 0.240336
  EX_hxan_e : 0.000462
  DM_5drib_c : 0.000277
  DM_4crsol_c : 0.000092

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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