MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : bmoco1gdp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (74 of 137: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 31
  Gene deletion: b2836 b3399 b1241 b0351 b4069 b2744 b3708 b2297 b2458 b2925 b2097 b1850 b3617 b0160 b1982 b1200 b2797 b3117 b1814 b4471 b4374 b0675 b2361 b2291 b0261 b0112 b0114 b1539 b2492 b0904 b1533   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.505440 (mmol/gDw/h)
  Minimum Production Rate : 0.241384 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 23.033891
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.079481
  EX_pi_e : 1.211704
  EX_so4_e : 1.092818
  EX_mobd_e : 0.241388
  EX_k_e : 0.098658
  EX_fe2_e : 0.008118
  EX_mg2_e : 0.004385
  EX_ca2_e : 0.002631
  EX_cl_e : 0.002631
  EX_cu2_e : 0.000358
  EX_mn2_e : 0.000349
  EX_zn2_e : 0.000172
  EX_ni2_e : 0.000163
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 47.992450
  EX_co2_e : 23.932443
  EX_h_e : 9.224176
  EX_ac_e : 3.855833
  Auxiliary production reaction : 0.241384
  DM_5drib_c : 0.030941
  DM_4crsol_c : 0.030714

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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