MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : coa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (20 of 83: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 30
  Gene deletion: b3399 b4382 b2502 b4384 b2744 b3708 b3008 b0871 b1982 b2797 b3117 b1814 b4471 b0675 b2361 b0261 b4381 b3915 b0452 b0114 b0886 b2366 b2492 b0904 b2578 b1533 b3927 b4141 b1798 b4209   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.770982 (mmol/gDw/h)
  Minimum Production Rate : 0.088103 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 24.766190
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.943261
  EX_pi_e : 1.008004
  EX_so4_e : 0.282252
  EX_k_e : 0.150490
  EX_fe3_e : 0.012383
  EX_mg2_e : 0.006688
  EX_ca2_e : 0.004013
  EX_cl_e : 0.004013
  EX_cu2_e : 0.000547
  EX_mn2_e : 0.000533
  EX_zn2_e : 0.000263
  EX_ni2_e : 0.000249
  EX_cobalt2_e : 0.000019

Product: (mmol/gDw/h)
  EX_h2o_e : 48.530121
  EX_co2_e : 26.502852
  EX_h_e : 7.360780
  Auxiliary production reaction : 0.088103
  DM_5drib_c : 0.000517
  DM_4crsol_c : 0.000172

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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