MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : coa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (24 of 83: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 31
  Gene deletion: b2836 b3553 b3399 b1241 b0351 b4069 b2502 b2744 b3708 b2297 b2458 b3617 b1238 b1982 b2797 b3117 b1814 b4471 b1033 b0675 b2361 b0261 b0507 b4381 b2406 b0112 b0114 b1539 b2492 b0904 b1533   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.518684 (mmol/gDw/h)
  Minimum Production Rate : 0.517353 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 990.925495
  EX_o2_e : 270.724112
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.223800
  EX_pi_e : 2.052385
  EX_so4_e : 0.647968
  EX_k_e : 0.101244
  EX_mg2_e : 0.004500
  EX_ca2_e : 0.002700
  EX_cl_e : 0.002700
  EX_cu2_e : 0.000368
  EX_mn2_e : 0.000358
  EX_zn2_e : 0.000177
  EX_ni2_e : 0.000168
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_fe3_e : 999.991669
  EX_h2o_e : 545.873230
  EX_co2_e : 22.349503
  EX_ac_e : 2.746728
  Auxiliary production reaction : 0.517353
  DM_oxam_c : 0.000580
  DM_5drib_c : 0.000348
  EX_glyclt_e : 0.000347
  DM_4crsol_c : 0.000116

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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