MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : coa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (53 of 83: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 34
  Gene deletion: b3399 b4382 b1241 b0351 b4069 b2744 b3708 b2297 b2458 b2883 b2797 b3117 b1814 b4471 b3616 b3589 b1623 b3665 b4381 b2406 b0112 b0452 b3654 b3714 b3664 b4064 b4464 b0114 b2366 b2492 b0904 b1533 b3662 b1518   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.520734 (mmol/gDw/h)
  Minimum Production Rate : 0.509993 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 20.799717
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.193837
  EX_pi_e : 2.032283
  EX_so4_e : 0.641124
  EX_k_e : 0.101644
  EX_fe2_e : 0.008364
  EX_mg2_e : 0.004517
  EX_ca2_e : 0.002710
  EX_cl_e : 0.002710
  EX_cu2_e : 0.000369
  EX_mn2_e : 0.000360
  EX_zn2_e : 0.000178
  EX_ni2_e : 0.000168
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 45.861987
  EX_co2_e : 22.420967
  EX_h_e : 9.061825
  EX_ac_e : 2.747133
  Auxiliary production reaction : 0.509993
  DM_mththf_c : 0.000233
  DM_5drib_c : 0.000117
  DM_4crsol_c : 0.000116

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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