MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dcamp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (40 of 77: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 27
  Gene deletion: b3399 b4069 b2744 b3708 b3008 b3115 b1849 b2296 b0160 b1982 b2797 b3117 b1814 b4471 b2440 b0675 b2361 b0261 b4381 b2406 b0452 b0114 b1539 b2492 b0904 b1533 b3927   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.736192 (mmol/gDw/h)
  Minimum Production Rate : 0.292546 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 24.280770
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.413599
  EX_pi_e : 1.002681
  EX_so4_e : 0.185388
  EX_k_e : 0.143699
  EX_fe2_e : 0.011824
  EX_mg2_e : 0.006386
  EX_ca2_e : 0.003832
  EX_cl_e : 0.003832
  EX_cu2_e : 0.000522
  EX_mn2_e : 0.000509
  EX_zn2_e : 0.000251
  EX_ni2_e : 0.000238
  EX_cobalt2_e : 0.000018

Product: (mmol/gDw/h)
  EX_h2o_e : 48.364537
  EX_co2_e : 24.828100
  EX_h_e : 9.240848
  EX_ac_e : 0.428601
  Auxiliary production reaction : 0.292546
  DM_5drib_c : 0.000494
  DM_4crsol_c : 0.000164
  EX_etha_e : 0.000059

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

---

Last updated: 21-Sep-2023
Contact