MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dctp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (52 of 73: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 39
  Gene deletion: b4467 b3399 b3942 b1732 b4069 b2744 b3115 b1849 b2296 b2926 b0160 b3844 b1004 b3713 b1109 b0046 b3236 b0937 b1982 b2210 b0675 b2361 b2291 b0261 b2799 b3945 b1602 b2913 b4381 b1727 b0114 b0529 b2492 b0904 b3927 b1380 b0606 b2285 b1010   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.386059 (mmol/gDw/h)
  Minimum Production Rate : 0.874428 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 34.863391
  EX_glc__D_e : 10.000000
  EX_nh4_e : 6.794422
  EX_pi_e : 2.995681
  EX_so4_e : 0.097217
  EX_k_e : 0.075356
  EX_fe2_e : 0.006201
  EX_mg2_e : 0.003349
  EX_ca2_e : 0.002009
  EX_cl_e : 0.002009
  EX_cu2_e : 0.000274
  EX_mn2_e : 0.000267
  EX_zn2_e : 0.000132
  EX_ni2_e : 0.000125

Product: (mmol/gDw/h)
  EX_h2o_e : 53.754762
  EX_co2_e : 35.151765
  EX_h_e : 4.648183
  Auxiliary production reaction : 0.874428
  EX_etoh_e : 0.339938
  EX_ac_e : 0.224758
  EX_hxan_e : 0.000432
  DM_5drib_c : 0.000259
  DM_4crsol_c : 0.000086

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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