MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dctp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (71 of 73: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 48
  Gene deletion: b3399 b3942 b1732 b1241 b0351 b4069 b2744 b1479 b2297 b2458 b2779 b0160 b3844 b3236 b2463 b3962 b1982 b0104 b3616 b3589 b2210 b4267 b1623 b3665 b0675 b2361 b1415 b3551 b1014 b3945 b2913 b4219 b1832 b1778 b4381 b2406 b0112 b0114 b0529 b2492 b0904 b1380 b0325 b1710 b2480 b0508 b1511 b4266   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.332517 (mmol/gDw/h)
  Minimum Production Rate : 1.045821 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 34.230868
  EX_glc__D_e : 10.000000
  EX_nh4_e : 7.267800
  EX_pi_e : 3.458212
  EX_so4_e : 0.083734
  EX_k_e : 0.064905
  EX_fe2_e : 0.005341
  EX_mg2_e : 0.002885
  EX_cl_e : 0.001731
  EX_ca2_e : 0.001731
  EX_cu2_e : 0.000236
  EX_mn2_e : 0.000230
  EX_zn2_e : 0.000113
  EX_ni2_e : 0.000107

Product: (mmol/gDw/h)
  EX_h2o_e : 53.933747
  EX_co2_e : 33.616318
  EX_h_e : 5.771170
  EX_ac_e : 1.121947
  Auxiliary production reaction : 1.045821
  EX_ade_e : 0.106096
  DM_mththf_c : 0.105872
  DM_oxam_c : 0.008701
  DM_5drib_c : 0.000223
  EX_dxylnt_e : 0.000222
  DM_4crsol_c : 0.000074

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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