MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dcyt_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (22 of 41: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 34
  Gene deletion: b3553 b1478 b1241 b4069 b4384 b3752 b2297 b2458 b2779 b2407 b1004 b3713 b1109 b0046 b3236 b1982 b1033 b0261 b0411 b2799 b3945 b1602 b4381 b3915 b0529 b2492 b0904 b1380 b2660 b1695 b0606 b2285 b1010 b4209   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.382590 (mmol/gDw/h)
  Minimum Production Rate : 1.046354 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 34.660145
  EX_glc__D_e : 10.000000
  EX_nh4_e : 7.272710
  EX_pi_e : 0.369048
  EX_so4_e : 0.096344
  EX_k_e : 0.074679
  EX_fe3_e : 0.006145
  EX_mg2_e : 0.003319
  EX_ca2_e : 0.001991
  EX_cl_e : 0.001991
  EX_cu2_e : 0.000271
  EX_mn2_e : 0.000264
  EX_zn2_e : 0.000130
  EX_ni2_e : 0.000124

Product: (mmol/gDw/h)
  EX_h2o_e : 51.859190
  EX_co2_e : 34.430796
  EX_h_e : 6.885044
  Auxiliary production reaction : 1.046354
  EX_ac_e : 0.222738
  EX_hxan_e : 0.000428
  DM_5drib_c : 0.000257
  DM_4crsol_c : 0.000085

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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