MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dcyt_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (37 of 41: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 44
  Gene deletion: b4467 b1478 b1241 b4069 b4384 b3752 b2297 b2458 b2926 b2407 b3844 b1004 b3713 b1109 b0046 b3236 b1638 b1982 b0477 b4139 b4014 b0261 b2976 b2799 b3945 b1602 b0507 b0153 b4381 b2406 b2975 b0114 b3603 b0584 b0529 b2492 b0904 b2954 b3029 b1380 b2660 b3662 b2285 b1009   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.411405 (mmol/gDw/h)
  Minimum Production Rate : 0.863316 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 34.847233
  EX_glc__D_e : 10.000000
  EX_nh4_e : 7.035393
  EX_pi_e : 0.396844
  EX_so4_e : 0.103600
  EX_k_e : 0.080303
  EX_mg2_e : 0.003569
  EX_fe2_e : 0.003395
  EX_fe3_e : 0.003212
  EX_cl_e : 0.002141
  EX_ca2_e : 0.002141
  EX_cu2_e : 0.000292
  EX_mn2_e : 0.000284
  EX_zn2_e : 0.000140
  EX_ni2_e : 0.000133
  EX_cobalt2_e : 0.000010

Product: (mmol/gDw/h)
  EX_h2o_e : 51.771896
  EX_co2_e : 34.861608
  EX_h_e : 6.615133
  Auxiliary production reaction : 0.863316
  EX_ac_e : 0.239514
  EX_ade_e : 0.000460
  DM_5drib_c : 0.000276
  DM_4crsol_c : 0.000092

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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