MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dgtp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (43 of 79: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 28
  Gene deletion: b4382 b4384 b3708 b3008 b0910 b3752 b0871 b2925 b2097 b2407 b3236 b1779 b2797 b3117 b1814 b4471 b3946 b2210 b0825 b3665 b0114 b2366 b2492 b0904 b1533 b3927 b3825 b2285   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.374742 (mmol/gDw/h)
  Minimum Production Rate : 0.064700 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 30.166042
  EX_glc__D_e : 10.000000
  EX_nh4_e : 4.371940
  EX_pi_e : 0.555579
  EX_so4_e : 0.094367
  EX_k_e : 0.073147
  EX_fe2_e : 0.006019
  EX_mg2_e : 0.003251
  EX_ca2_e : 0.001951
  EX_cl_e : 0.001951
  EX_cu2_e : 0.000266
  EX_mn2_e : 0.000259
  EX_zn2_e : 0.000128
  EX_ni2_e : 0.000121

Product: (mmol/gDw/h)
  EX_h2o_e : 44.240972
  EX_co2_e : 28.169977
  EX_h_e : 8.905417
  EX_pyr_e : 5.266780
  Auxiliary production reaction : 0.064700
  EX_ade_e : 0.000252
  DM_5drib_c : 0.000084
  DM_4crsol_c : 0.000084

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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