MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dgtp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (76 of 79: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 44
  Gene deletion: b3553 b3399 b4069 b2744 b3708 b2297 b2458 b2926 b3844 b1004 b3713 b1109 b0046 b3236 b0937 b2797 b3117 b1814 b4471 b2210 b1033 b2799 b3945 b1602 b2913 b4381 b2406 b3654 b3714 b3664 b1727 b4064 b4464 b0114 b2366 b2492 b0904 b1533 b3821 b1380 b1517 b0606 b2285 b1011   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.467938 (mmol/gDw/h)
  Minimum Production Rate : 0.209884 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 36.807201
  EX_glc__D_e : 10.000000
  EX_nh4_e : 6.153350
  EX_pi_e : 1.081029
  EX_so4_e : 0.117836
  EX_k_e : 0.091338
  EX_fe2_e : 0.007516
  EX_mg2_e : 0.004059
  EX_cl_e : 0.002436
  EX_ca2_e : 0.002436
  EX_cu2_e : 0.000332
  EX_mn2_e : 0.000323
  EX_zn2_e : 0.000160
  EX_ni2_e : 0.000151
  EX_cobalt2_e : 0.000012

Product: (mmol/gDw/h)
  EX_h2o_e : 52.651767
  EX_co2_e : 37.524184
  EX_h_e : 5.251919
  EX_etoh_e : 0.280781
  EX_ac_e : 0.272427
  Auxiliary production reaction : 0.209884
  EX_xan_e : 0.012559
  DM_mththf_c : 0.000210
  DM_5drib_c : 0.000105
  DM_4crsol_c : 0.000104

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
Contact