MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : duri_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (27 of 50: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 31
  Gene deletion: b2836 b3399 b4069 b2744 b3708 b2930 b4232 b3697 b3925 b3115 b1849 b2296 b0160 b1982 b2797 b3117 b1814 b4471 b3946 b0825 b0675 b2361 b0261 b4381 b2406 b0114 b2366 b2492 b0904 b1533 b3927   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.758306 (mmol/gDw/h)
  Minimum Production Rate : 0.055940 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 24.525759
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.377816
  EX_pi_e : 0.731467
  EX_so4_e : 0.190957
  EX_k_e : 0.148016
  EX_fe2_e : 0.012179
  EX_mg2_e : 0.006578
  EX_cl_e : 0.003947
  EX_ca2_e : 0.003947
  EX_cu2_e : 0.000538
  EX_mn2_e : 0.000524
  EX_zn2_e : 0.000259
  EX_ni2_e : 0.000245
  EX_cobalt2_e : 0.000019

Product: (mmol/gDw/h)
  EX_h2o_e : 46.486620
  EX_co2_e : 25.973623
  EX_h_e : 8.354756
  EX_ac_e : 1.122671
  DM_oxam_c : 0.076292
  Auxiliary production reaction : 0.055940
  DM_5drib_c : 0.000171
  DM_4crsol_c : 0.000169

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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