MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : duri_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (43 of 50: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 40
  Gene deletion: b3399 b4382 b4384 b2744 b3708 b3008 b0871 b0160 b0583 b1982 b2797 b3117 b1814 b4471 b0675 b2361 b2291 b0261 b3709 b2406 b3161 b0112 b2975 b0114 b3603 b0886 b0509 b3125 b1539 b2492 b0904 b2578 b1533 b3927 b3821 b0508 b1473 b1600 b4141 b1798   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.742742 (mmol/gDw/h)
  Minimum Production Rate : 0.507319 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 23.838042
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.039089
  EX_pi_e : 0.716453
  EX_so4_e : 0.187037
  EX_k_e : 0.144978
  EX_fe2_e : 0.011929
  EX_mg2_e : 0.006443
  EX_ca2_e : 0.003866
  EX_cl_e : 0.003866
  EX_cu2_e : 0.000527
  EX_mn2_e : 0.000513
  EX_zn2_e : 0.000253
  EX_ni2_e : 0.000240
  EX_cobalt2_e : 0.000019

Product: (mmol/gDw/h)
  EX_h2o_e : 47.585385
  EX_co2_e : 24.915702
  EX_h_e : 7.845048
  Auxiliary production reaction : 0.507319
  DM_oxam_c : 0.002900
  DM_5drib_c : 0.002567
  DM_4crsol_c : 0.002234

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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