MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : dutp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (23 of 79: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 25
  Gene deletion: b3399 b1241 b0351 b4069 b2744 b2297 b2458 b2883 b3616 b3589 b1623 b3665 b0411 b4381 b0112 b3654 b3714 b3664 b0114 b0529 b2492 b0904 b2954 b1511 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.524623 (mmol/gDw/h)
  Minimum Production Rate : 1.550995 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 19.945067
  EX_glc__D_e : 10.000000
  EX_nh4_e : 9.690404
  EX_pi_e : 5.159040
  EX_so4_e : 0.132111
  EX_k_e : 0.102403
  EX_fe2_e : 0.008426
  EX_mg2_e : 0.004551
  EX_cl_e : 0.002731
  EX_ca2_e : 0.002731
  EX_cu2_e : 0.000372
  EX_mn2_e : 0.000363
  EX_zn2_e : 0.000179
  EX_ni2_e : 0.000169
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 49.863386
  EX_co2_e : 19.282995
  EX_h_e : 6.048404
  Auxiliary production reaction : 1.550995
  EX_thymd_e : 0.461266
  EX_ac_e : 0.305428
  DM_mththf_c : 0.000235
  DM_5drib_c : 0.000118
  DM_4crsol_c : 0.000117

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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