MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : gdpddman_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (81 of 82: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 57
  Gene deletion: b1478 b3399 b3942 b1732 b0474 b2518 b1241 b0351 b4069 b2502 b2744 b1479 b0871 b2297 b2458 b2925 b2097 b3236 b2690 b2463 b3962 b1982 b3616 b3589 b2210 b4267 b0675 b2361 b1415 b3551 b1014 b0822 b2913 b4219 b1832 b1778 b4381 b2406 b0112 b2868 b0114 b0509 b3125 b0755 b3612 b0529 b2492 b0904 b1380 b0325 b1710 b2480 b4042 b4266 b1518 b1206 b4209   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.257603 (mmol/gDw/h)
  Minimum Production Rate : 0.408973 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 28.896540
  EX_glc__D_e : 10.000000
  EX_nh4_e : 5.167197
  EX_pi_e : 1.066431
  EX_so4_e : 0.064869
  EX_k_e : 0.050282
  EX_mg2_e : 0.002235
  EX_fe2_e : 0.002126
  EX_fe3_e : 0.002011
  EX_ca2_e : 0.001341
  EX_cl_e : 0.001341
  EX_cu2_e : 0.000183
  EX_mn2_e : 0.000178
  EX_zn2_e : 0.000088
  EX_ni2_e : 0.000083

Product: (mmol/gDw/h)
  EX_h2o_e : 44.008153
  EX_co2_e : 25.701163
  EX_h_e : 9.815858
  EX_pyr_e : 4.605275
  EX_ac_e : 1.274278
  Auxiliary production reaction : 0.408973
  EX_hxan_e : 0.078321
  EX_mththf_e : 0.078148
  EX_xan_e : 0.006740
  DM_5drib_c : 0.000173
  EX_dxylnt_e : 0.000172
  DM_4crsol_c : 0.000057

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

---

Last updated: 21-Sep-2023
Contact