MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : gdpfuc_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (49 of 74: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 30
  Gene deletion: b4382 b0474 b2518 b4384 b3708 b3008 b3752 b0871 b2407 b1779 b1982 b2797 b3117 b1814 b4471 b3665 b0261 b2406 b0114 b0886 b1539 b2492 b0904 b2578 b1533 b3927 b3821 b4141 b1798 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.683765 (mmol/gDw/h)
  Minimum Production Rate : 0.156948 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 28.353017
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.173174
  EX_pi_e : 0.973461
  EX_so4_e : 0.172186
  EX_k_e : 0.133466
  EX_fe2_e : 0.010982
  EX_mg2_e : 0.005932
  EX_cl_e : 0.003559
  EX_ca2_e : 0.003559
  EX_cu2_e : 0.000485
  EX_mn2_e : 0.000472
  EX_zn2_e : 0.000233
  EX_ni2_e : 0.000221
  EX_cobalt2_e : 0.000017

Product: (mmol/gDw/h)
  EX_h2o_e : 49.679332
  EX_co2_e : 29.418055
  EX_h_e : 6.757378
  Auxiliary production reaction : 0.156948
  EX_ade_e : 0.000765
  DM_5drib_c : 0.000459
  DM_4crsol_c : 0.000152

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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