MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : glyc__R_e
List of minimal gene deletion strategies (Download)

Gene deletion strategy (12 of 83: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 29
  Gene deletion: b1241 b0351 b4069 b4384 b3708 b2297 b2458 b3617 b0030 b2407 b1238 b3124 b1982 b2797 b3117 b1814 b4471 b0261 b4381 b2406 b0112 b0452 b0114 b2366 b2492 b0904 b1533 b0514 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.524606 (mmol/gDw/h)
  Minimum Production Rate : 0.357000 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 21.691990
  EX_glc__D_e : 10.000000
  EX_nh4_e : 6.379936
  EX_pi_e : 0.506038
  EX_so4_e : 0.132106
  EX_k_e : 0.102399
  EX_fe2_e : 0.008426
  EX_mg2_e : 0.004551
  EX_ca2_e : 0.002731
  EX_cl_e : 0.002731
  EX_cu2_e : 0.000372
  EX_mn2_e : 0.000363
  EX_zn2_e : 0.000179
  EX_ni2_e : 0.000169
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 40.411884
  EX_co2_e : 22.989071
  EX_h_e : 8.812677
  EX_ac_e : 2.206916
  DM_oxam_c : 0.714236
  DM_5drib_c : 0.714001
  DM_4crsol_c : 0.713766
  EX_glyc__R_e : 0.357000

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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