MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : gmp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (56 of 102: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 25
  Gene deletion: b4384 b2744 b3708 b3008 b3752 b0871 b2407 b1779 b1982 b2797 b3117 b1814 b4471 b0261 b4381 b0114 b0529 b1539 b2492 b0904 b2947 b1533 b3927 b3821 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.694493 (mmol/gDw/h)
  Minimum Production Rate : 0.159411 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 28.894628
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.297515
  EX_pi_e : 0.829322
  EX_so4_e : 0.174887
  EX_k_e : 0.135560
  EX_fe2_e : 0.011154
  EX_mg2_e : 0.006025
  EX_ca2_e : 0.003615
  EX_cl_e : 0.003615
  EX_cu2_e : 0.000492
  EX_mn2_e : 0.000480
  EX_zn2_e : 0.000237
  EX_ni2_e : 0.000224
  EX_cobalt2_e : 0.000017

Product: (mmol/gDw/h)
  EX_h2o_e : 50.151170
  EX_co2_e : 29.898613
  EX_h_e : 7.178329
  Auxiliary production reaction : 0.159411
  DM_5drib_c : 0.000466
  DM_4crsol_c : 0.000155

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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