MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : gsn_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (79 of 79: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 49
  Gene deletion: b4467 b3399 b4269 b0493 b3588 b3003 b3011 b4384 b2744 b2930 b4232 b3697 b3925 b0871 b2779 b1004 b3713 b1109 b0046 b3236 b2883 b1638 b3908 b1656 b1982 b4139 b3946 b0825 b0675 b2361 b0261 b2799 b3945 b1602 b2342 b3845 b2913 b4381 b2868 b1727 b4064 b4464 b0114 b0529 b2492 b0904 b1380 b3662 b2285   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.347896 (mmol/gDw/h)
  Minimum Production Rate : 0.009103 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 30.300906
  EX_glc__D_e : 10.000000
  EX_nh4_e : 4.056516
  EX_pi_e : 0.335583
  EX_so4_e : 0.087607
  EX_k_e : 0.067907
  EX_fe3_e : 0.005588
  EX_mg2_e : 0.003018
  EX_ca2_e : 0.001811
  EX_cl_e : 0.001811
  EX_cu2_e : 0.000247
  EX_mn2_e : 0.000240
  EX_zn2_e : 0.000119
  EX_ni2_e : 0.000112

Product: (mmol/gDw/h)
  EX_h2o_e : 43.109150
  EX_co2_e : 28.212092
  EX_h_e : 9.138465
  EX_pyr_e : 5.357043
  EX_3hpp_e : 0.279958
  EX_gly_e : 0.251805
  Auxiliary production reaction : 0.009103
  EX_ade_e : 0.000389
  DM_5drib_c : 0.000233
  DM_4crsol_c : 0.000078

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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