MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : imacp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (49 of 103: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 33
  Gene deletion: b4467 b1478 b1241 b4069 b2297 b2458 b2779 b3844 b1004 b3713 b1109 b0046 b3236 b0937 b0411 b2799 b3945 b1602 b4381 b3654 b3714 b3664 b4064 b4464 b0529 b2492 b0904 b1380 b2660 b1517 b0606 b2285 b1010   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.394167 (mmol/gDw/h)
  Minimum Production Rate : 1.239475 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 35.703157
  EX_glc__D_e : 10.000000
  EX_nh4_e : 6.735917
  EX_pi_e : 1.619691
  EX_so4_e : 0.099259
  EX_k_e : 0.076939
  EX_fe2_e : 0.006331
  EX_mg2_e : 0.003419
  EX_ca2_e : 0.002052
  EX_cl_e : 0.002052
  EX_cu2_e : 0.000279
  EX_mn2_e : 0.000272
  EX_zn2_e : 0.000134
  EX_ni2_e : 0.000127

Product: (mmol/gDw/h)
  EX_h2o_e : 53.770282
  EX_co2_e : 35.924578
  EX_h_e : 6.330189
  Auxiliary production reaction : 1.239475
  EX_ac_e : 0.229478
  DM_mththf_c : 0.000177
  DM_5drib_c : 0.000089
  DM_4crsol_c : 0.000088

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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