MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : imp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (52 of 81: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 28
  Gene deletion: b2836 b3399 b4382 b4069 b4384 b2744 b3708 b0512 b2297 b2458 b2779 b0030 b2407 b3236 b1982 b2797 b3117 b1814 b4471 b2210 b0114 b2366 b0529 b2492 b0904 b1533 b3927 b1511   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.657068 (mmol/gDw/h)
  Minimum Production Rate : 0.191160 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 992.803097
  EX_o2_e : 279.424844
  EX_glc__D_e : 10.000000
  EX_nh4_e : 7.862690
  EX_pi_e : 0.824972
  EX_so4_e : 0.165463
  EX_k_e : 0.128255
  EX_mg2_e : 0.005700
  EX_ca2_e : 0.003420
  EX_cl_e : 0.003420
  EX_cu2_e : 0.000466
  EX_mn2_e : 0.000454
  EX_zn2_e : 0.000224
  EX_ni2_e : 0.000212
  EX_cobalt2_e : 0.000016

Product: (mmol/gDw/h)
  EX_fe3_e : 999.989447
  EX_h2o_e : 549.802388
  EX_co2_e : 30.350454
  EX_ac_e : 0.382536
  Auxiliary production reaction : 0.191160
  EX_alltn_e : 0.000442
  EX_mththf_e : 0.000294
  DM_5drib_c : 0.000148
  DM_4crsol_c : 0.000147

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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