MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : malcoa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (65 of 86: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 54
  Gene deletion: b1478 b1241 b0351 b4069 b4384 b3708 b2297 b2458 b2926 b0030 b2407 b1238 b3962 b1982 b0477 b4139 b0104 b2797 b3117 b1814 b4471 b3616 b3589 b4267 b1415 b1014 b4014 b0261 b2976 b1701 b1805 b3945 b0507 b3709 b4381 b2406 b3161 b0112 b2975 b0114 b3603 b2366 b2492 b0904 b2954 b3035 b1533 b1380 b0325 b0508 b1473 b4266 b3662 b1813   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.316954 (mmol/gDw/h)
  Minimum Production Rate : 0.085405 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 33.347250
  EX_glc__D_e : 10.000000
  EX_nh4_e : 4.798907
  EX_so4_e : 0.942857
  EX_pi_e : 0.561952
  EX_k_e : 0.061867
  EX_fe2_e : 0.005091
  EX_mg2_e : 0.002750
  EX_ca2_e : 0.001650
  EX_cl_e : 0.001650
  EX_cu2_e : 0.000225
  EX_mn2_e : 0.000219
  EX_zn2_e : 0.000108
  EX_ni2_e : 0.000102

Product: (mmol/gDw/h)
  EX_h2o_e : 47.686541
  EX_co2_e : 35.967161
  EX_h_e : 5.018676
  EX_ac_e : 2.541468
  EX_met__L_e : 0.777637
  Auxiliary production reaction : 0.085405
  DM_oxam_c : 0.000355
  DM_5drib_c : 0.000213
  EX_dxylnt_e : 0.000212
  DM_4crsol_c : 0.000071

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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