MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : mmcoa__S_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (12 of 85: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 30
  Gene deletion: b2836 b4382 b4384 b3708 b3008 b3752 b0871 b2407 b2797 b3117 b1814 b4471 b3665 b2406 b3654 b3714 b3664 b0114 b0886 b2366 b2492 b0904 b2578 b1533 b3927 b4141 b1798 b3662 b1518 b4209   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.766590 (mmol/gDw/h)
  Minimum Production Rate : 0.088435 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 24.640778
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.898152
  EX_pi_e : 1.004762
  EX_so4_e : 0.281478
  EX_k_e : 0.149633
  EX_mg2_e : 0.006650
  EX_fe2_e : 0.006327
  EX_fe3_e : 0.005986
  EX_ca2_e : 0.003990
  EX_cl_e : 0.003990
  EX_cu2_e : 0.000544
  EX_mn2_e : 0.000530
  EX_zn2_e : 0.000261
  EX_ni2_e : 0.000248
  EX_cobalt2_e : 0.000019

Product: (mmol/gDw/h)
  EX_h2o_e : 48.420075
  EX_co2_e : 26.322410
  EX_h_e : 7.403460
  Auxiliary production reaction : 0.088435
  EX_mththf_e : 0.000343
  DM_5drib_c : 0.000172
  DM_4crsol_c : 0.000171

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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