MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : mnl1p_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (27 of 80: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 35
  Gene deletion: b4467 b1478 b0474 b2518 b1241 b0351 b4069 b3115 b1849 b2296 b2779 b1004 b3713 b1109 b0046 b3236 b3616 b3589 b2799 b3945 b1602 b2913 b4381 b3915 b4064 b4464 b0529 b2492 b0904 b1380 b1518 b0606 b2285 b1010 b4209   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.458792 (mmol/gDw/h)
  Minimum Production Rate : 0.439002 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 994.385291
  EX_o2_e : 284.552011
  EX_glc__D_e : 10.000000
  EX_nh4_e : 4.956456
  EX_pi_e : 0.881555
  EX_so4_e : 0.115533
  EX_k_e : 0.089553
  EX_mg2_e : 0.003980
  EX_ca2_e : 0.002388
  EX_cl_e : 0.002388
  EX_cu2_e : 0.000325
  EX_mn2_e : 0.000317
  EX_zn2_e : 0.000156
  EX_ni2_e : 0.000148
  EX_cobalt2_e : 0.000011

Product: (mmol/gDw/h)
  EX_fe3_e : 999.992631
  EX_h2o_e : 547.866478
  EX_co2_e : 35.751654
  EX_ac_e : 1.390235
  Auxiliary production reaction : 0.439002
  EX_ade_e : 0.000309
  DM_mththf_c : 0.000206
  DM_5drib_c : 0.000103
  DM_4crsol_c : 0.000102

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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