MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : nad_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (37 of 79: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 32
  Gene deletion: b1241 b0351 b4069 b4384 b2744 b3708 b2297 b2458 b3617 b0030 b2407 b1982 b2797 b3117 b1814 b4471 b4374 b2361 b2291 b0261 b0507 b2406 b0112 b2975 b0114 b3603 b1539 b2492 b0904 b1533 b3821 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.500301 (mmol/gDw/h)
  Minimum Production Rate : 0.748545 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 21.119740
  EX_nh4_e : 10.643579
  EX_glc__D_e : 10.000000
  EX_pi_e : 1.979683
  EX_so4_e : 0.125986
  EX_k_e : 0.097655
  EX_fe2_e : 0.008035
  EX_mg2_e : 0.004340
  EX_ca2_e : 0.002604
  EX_cl_e : 0.002604
  EX_cu2_e : 0.000355
  EX_mn2_e : 0.000346
  EX_zn2_e : 0.000171
  EX_ni2_e : 0.000162
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 48.873149
  EX_co2_e : 19.941030
  EX_h_e : 9.493386
  EX_ac_e : 1.901125
  Auxiliary production reaction : 0.748545
  DM_oxam_c : 0.000560
  DM_5drib_c : 0.000336
  DM_4crsol_c : 0.000112

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

---

Last updated: 21-Sep-2023
Contact