MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : pgp140_p
List of minimal gene deletion strategies (Download)

Gene deletion strategy (67 of 74: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 34
  Gene deletion: b4467 b2836 b2242 b0474 b2518 b3831 b3752 b2781 b1004 b3713 b1109 b0046 b1612 b1611 b4122 b1759 b2210 b4161 b4138 b4123 b0621 b4381 b2406 b0306 b3605 b2492 b0904 b2197 b3028 b3918 b4042 b1206 b0221 b2285   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.464389 (mmol/gDw/h)
  Minimum Production Rate : 0.245265 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 25.902314
  EX_glc__D_e : 10.000000
  EX_nh4_e : 5.673999
  EX_pi_e : 0.938481
  EX_so4_e : 0.116942
  EX_k_e : 0.090645
  EX_fe2_e : 0.007459
  EX_mg2_e : 0.004029
  EX_ca2_e : 0.002417
  EX_cl_e : 0.002417
  EX_cu2_e : 0.000329
  EX_mn2_e : 0.000321
  EX_zn2_e : 0.000158
  EX_ni2_e : 0.000150
  EX_cobalt2_e : 0.000012

Product: (mmol/gDw/h)
  EX_h2o_e : 44.475535
  EX_co2_e : 29.345658
  EX_h_e : 5.648886
  EX_succ_e : 0.484261
  EX_ura_e : 0.329320
  Auxiliary production reaction : 0.245265
  DM_5drib_c : 0.000104
  DM_4crsol_c : 0.000104

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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