MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : pgp161_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (62 of 68: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 27
  Gene deletion: b1054 b3831 b2744 b1278 b3614 b0910 b3752 b4152 b2781 b3844 b1612 b1611 b4122 b1759 b4374 b2361 b2291 b4138 b4123 b0621 b0153 b2406 b0452 b0584 b2197 b3918 b1206   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.559591 (mmol/gDw/h)
  Minimum Production Rate : 0.295455 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 17.439084
  EX_glc__D_e : 10.000000
  EX_nh4_e : 6.837197
  EX_pi_e : 1.130694
  EX_so4_e : 0.140916
  EX_k_e : 0.109228
  EX_fe2_e : 0.008988
  EX_mg2_e : 0.004854
  EX_ca2_e : 0.002913
  EX_cl_e : 0.002913
  EX_cu2_e : 0.000397
  EX_mn2_e : 0.000387
  EX_zn2_e : 0.000191
  EX_ni2_e : 0.000181
  EX_cobalt2_e : 0.000014

Product: (mmol/gDw/h)
  EX_h2o_e : 40.704829
  EX_co2_e : 21.882613
  EX_h_e : 6.807026
  EX_succ_e : 0.583537
  EX_ura_e : 0.396833
  Auxiliary production reaction : 0.295455
  DM_5drib_c : 0.000126
  DM_4crsol_c : 0.000125

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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