MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : pnto__R_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (43 of 79: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 34
  Gene deletion: b3399 b4069 b2744 b3708 b3008 b3115 b1849 b2296 b2925 b2097 b0160 b2690 b1982 b2797 b3117 b1814 b4471 b4374 b0675 b2361 b2291 b0261 b1701 b1805 b3945 b2868 b0114 b2366 b2492 b0904 b2578 b1533 b3927 b0594   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.728279 (mmol/gDw/h)
  Minimum Production Rate : 0.016121 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 992.381591
  EX_o2_e : 273.899913
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.794916
  EX_pi_e : 0.702502
  EX_so4_e : 0.638080
  EX_k_e : 0.142155
  EX_mg2_e : 0.006318
  EX_cl_e : 0.003791
  EX_ca2_e : 0.003791
  EX_cu2_e : 0.000516
  EX_mn2_e : 0.000503
  EX_zn2_e : 0.000248
  EX_ni2_e : 0.000235
  EX_cobalt2_e : 0.000018

Product: (mmol/gDw/h)
  EX_fe3_e : 999.988303
  EX_h2o_e : 547.011044
  EX_co2_e : 25.925930
  EX_ac_e : 0.878679
  EX_cgly_e : 0.454685
  Auxiliary production reaction : 0.016121
  EX_ade_e : 0.000815
  DM_5drib_c : 0.000489
  DM_4crsol_c : 0.000162

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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