MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : ppcoa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (37 of 117: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 31
  Gene deletion: b4382 b1241 b0351 b4069 b4384 b3708 b3115 b1849 b2296 b3617 b0030 b2407 b1982 b2797 b3117 b1814 b4471 b3449 b0261 b0507 b2406 b0112 b0452 b2975 b0114 b3603 b2366 b2492 b0904 b1533 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.502485 (mmol/gDw/h)
  Minimum Production Rate : 0.501196 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 20.198406
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.935731
  EX_pi_e : 1.988287
  EX_so4_e : 0.627731
  EX_k_e : 0.098082
  EX_fe2_e : 0.008070
  EX_mg2_e : 0.004359
  EX_ca2_e : 0.002615
  EX_cl_e : 0.002615
  EX_cu2_e : 0.000356
  EX_mn2_e : 0.000347
  EX_zn2_e : 0.000171
  EX_ni2_e : 0.000162
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 45.316069
  EX_co2_e : 22.021782
  EX_h_e : 8.783028
  EX_ac_e : 2.661281
  Auxiliary production reaction : 0.501196
  DM_oxam_c : 0.000562
  DM_5drib_c : 0.000337
  DM_4crsol_c : 0.000112

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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