MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : prbamp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (41 of 47: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 38
  Gene deletion: b2836 b4382 b4269 b0493 b3588 b3003 b3011 b1241 b0351 b4384 b3708 b3008 b3752 b0871 b2925 b2097 b3617 b2407 b2690 b1982 b2797 b3117 b1814 b4471 b3665 b0261 b3945 b2406 b0112 b0114 b1539 b2492 b0904 b2578 b1533 b4141 b1798 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.580167 (mmol/gDw/h)
  Minimum Production Rate : 0.375308 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 22.286139
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.793235
  EX_pi_e : 1.310249
  EX_so4_e : 0.793795
  EX_k_e : 0.113244
  EX_fe2_e : 0.009318
  EX_mg2_e : 0.005033
  EX_ca2_e : 0.003020
  EX_cl_e : 0.003020
  EX_cu2_e : 0.000411
  EX_mn2_e : 0.000401
  EX_zn2_e : 0.000198
  EX_ni2_e : 0.000187
  EX_cobalt2_e : 0.000015

Product: (mmol/gDw/h)
  EX_h2o_e : 46.443519
  EX_co2_e : 24.566720
  EX_h_e : 6.562893
  EX_acald_e : 2.021425
  EX_cys__L_e : 0.647697
  Auxiliary production reaction : 0.375308
  EX_ade_e : 0.000649
  DM_5drib_c : 0.000389
  DM_4crsol_c : 0.000129

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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