MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : prbatp_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (47 of 47: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 50
  Gene deletion: b4467 b1478 b0238 b0125 b0474 b2518 b1241 b3831 b4069 b4384 b2744 b2297 b2458 b2779 b0030 b3844 b1004 b3713 b1109 b0046 b3236 b2883 b1638 b3908 b1656 b1982 b0477 b4139 b2498 b0261 b0411 b2799 b3945 b1602 b0507 b0153 b0590 b2868 b4064 b4464 b2975 b3603 b0529 b2492 b0904 b2954 b1380 b2660 b2285 b1009   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.445041 (mmol/gDw/h)
  Minimum Production Rate : 0.225813 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_o2_e : 37.378043
  EX_glc__D_e : 10.000000
  EX_nh4_e : 5.999249
  EX_pi_e : 1.332542
  EX_so4_e : 0.112070
  EX_k_e : 0.086869
  EX_mg2_e : 0.003861
  EX_fe2_e : 0.003673
  EX_fe3_e : 0.003475
  EX_ca2_e : 0.002316
  EX_cl_e : 0.002316
  EX_cu2_e : 0.000316
  EX_mn2_e : 0.000308
  EX_zn2_e : 0.000152
  EX_ni2_e : 0.000144
  EX_cobalt2_e : 0.000011

Product: (mmol/gDw/h)
  EX_h2o_e : 53.162153
  EX_co2_e : 37.732313
  EX_h_e : 5.123649
  EX_ac_e : 0.289742
  Auxiliary production reaction : 0.225813
  EX_urea_e : 0.030645
  EX_ade_e : 0.000498
  DM_5drib_c : 0.000299
  DM_4crsol_c : 0.000099

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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