MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : preq0_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (34 of 51: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 27
  Gene deletion: b4269 b0493 b3588 b3003 b3011 b1241 b0351 b4384 b2744 b0871 b2779 b3617 b0030 b2407 b1982 b0261 b0411 b0507 b4381 b0112 b2975 b0114 b3603 b0529 b2492 b0904 b3662   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.512118 (mmol/gDw/h)
  Minimum Production Rate : 0.606793 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 23.890560
  EX_nh4_e : 10.681321
  EX_glc__D_e : 10.000000
  EX_pi_e : 0.493992
  EX_so4_e : 0.128961
  EX_k_e : 0.099962
  EX_fe2_e : 0.008225
  EX_mg2_e : 0.004443
  EX_ca2_e : 0.002666
  EX_cl_e : 0.002666
  EX_cu2_e : 0.000363
  EX_mn2_e : 0.000354
  EX_zn2_e : 0.000175
  EX_ni2_e : 0.000165
  EX_cobalt2_e : 0.000013

Product: (mmol/gDw/h)
  EX_h2o_e : 50.090855
  EX_co2_e : 22.936713
  EX_h_e : 9.856609
  EX_thymd_e : 1.057979
  Auxiliary production reaction : 0.606793
  EX_acald_e : 0.606793
  DM_oxam_c : 0.000573
  DM_5drib_c : 0.000344
  DM_4crsol_c : 0.000114

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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