MetNetComp Database [1] / Minimal gene deletions

Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : ps161_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (61 of 76: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 25
  Gene deletion: b4382 b4384 b2744 b1278 b3614 b0910 b4152 b2781 b1612 b1611 b4122 b1759 b4374 b0675 b4138 b4123 b0621 b2406 b3915 b0452 b2197 b3918 b1206 b3546 b4209   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.563498 (mmol/gDw/h)
  Minimum Production Rate : 0.297053 (mmol/gDw/h)

 Substrate: (mmol/gDw/h)
  EX_fe2_e : 1000.000000
  EX_h_e : 992.838876
  EX_o2_e : 267.460878
  EX_glc__D_e : 10.000000
  EX_nh4_e : 7.181990
  EX_pi_e : 0.840606
  EX_so4_e : 0.141900
  EX_k_e : 0.109991
  EX_mg2_e : 0.004888
  EX_ca2_e : 0.002933
  EX_cl_e : 0.002933
  EX_cu2_e : 0.000400
  EX_mn2_e : 0.000389
  EX_zn2_e : 0.000192
  EX_ni2_e : 0.000182
  EX_cobalt2_e : 0.000014

Product: (mmol/gDw/h)
  EX_fe3_e : 999.990950
  EX_h2o_e : 540.877976
  EX_co2_e : 21.634132
  EX_succ_e : 0.587611
  EX_ura_e : 0.399603
  Auxiliary production reaction : 0.297053
  DM_5drib_c : 0.000127
  DM_4crsol_c : 0.000126

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

Last updated: 21-Sep-2023
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