MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iML1515 [2].
Target metabolite : sbzcoa_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (16 of 83: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 39
  Gene deletion: b3553 b3399 b4382 b1241 b0351 b4069 b2502 b2744 b3708 b2297 b2458 b3617 b1982 b2797 b3117 b1814 b4471 b1033 b0595 b0675 b2361 b4014 b0261 b2976 b0507 b3709 b4381 b2406 b3161 b0112 b0114 b2366 b2492 b0904 b1533 b3821 b1473 b4141 b1798   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.394448 (mmol/gDw/h)
  Minimum Production Rate : 0.579848 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_o2_e : 18.509562
  EX_glc__D_e : 10.000000
  EX_nh4_e : 8.329709
  EX_pi_e : 2.120032
  EX_so4_e : 0.679178
  EX_k_e : 0.076994
  EX_fe2_e : 0.006335
  EX_mg2_e : 0.003422
  EX_cl_e : 0.002053
  EX_ca2_e : 0.002053
  EX_cu2_e : 0.000280
  EX_mn2_e : 0.000273
  EX_zn2_e : 0.000135
  EX_ni2_e : 0.000127

Product: (mmol/gDw/h)
  EX_h2o_e : 45.428856
  EX_co2_e : 19.914983
  EX_h_e : 8.623853
  EX_ac_e : 2.658322
  Auxiliary production reaction : 0.579848
  DM_oxam_c : 0.010763
  DM_5drib_c : 0.000265
  EX_glyclt_e : 0.000264
  DM_4crsol_c : 0.000088

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 21-Sep-2023
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