MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iMM904 [2].
Target metabolite : sql_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (38 of 69: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 20
  Gene deletion: YOL086C YDL168W YMR303C YGL256W YBR145W YOR374W YER073W YAL060W YOR120W YLL043W YDR300C YMR250W YDL171C YER065C YLR348C YKL085W YKL148C YDR178W YDL085W YMR145C   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.154444 (mmol/gDw/h)
  Minimum Production Rate : 0.015865 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_glc__D_e : 10.000000
  EX_o2_e : 2.000000
  EX_nh4_e : 0.882963
  EX_pi_e : 0.030533
  EX_so4_e : 0.011938

Product: (mmol/gDw/h)
  EX_h_e : 22.944200
  EX_h2o_e : 11.378047
  EX_succ_e : 9.437258
  EX_acald_e : 4.800583
  EX_ac_e : 3.283477
  Auxiliary production reaction : 0.015865
  EX_lys__L_e : 0.009313

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].
  3. Select "Data > Load reaction data" and apply the downloaded file.

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

---

Last updated: 27-Sep-2023
Contact