MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iPC815 [2].
Target metabolite : apg120_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (29 of 34: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 27
  Gene deletion: YPO1384 YPO4078 YPO3825 YPO3937 YPO0918 YPO3428 YPO0348 YPO3992 YPO0347 YPO3831 YPO0460 YPO3202 YPO3516 YPO1145 YPO3014 YPO0670 YPO2303 YPO3914 YPO0440 YPO3376 YPO1851 YPO3418 YPO2370 YPO0914 YPO2836 YPO2567 YPO1171   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.097028 (mmol/gDw/h)
  Minimum Production Rate : 0.091991 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_h_e : 9.210925
  EX_cit_e : 7.400000
  EX_o2_e : 4.384814
  EX_gly_e : 2.200000
  EX_cys__L_e : 1.000000
  EX_glc__D_e : 0.243619
  EX_pi_e : 0.171437
  EX_phe__L_e : 0.018435
  EX_met__L_e : 0.013584
  EX_tyr__L_e : 0.012614

Product: (mmol/gDw/h)
  EX_co2_e : 14.846558
  EX_succ_e : 7.400000
  EX_h2o_e : 5.510033
  EX_nh4_e : 2.056509
  EX_h2s_e : 0.992911
  EX_ac_e : 0.410000
  EX_glyald_e : 0.092509
  Auxiliary production reaction : 0.091998

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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