MetNetComp Database [1] / Minimal gene deletions Minimal gene deletions for simulation-based growth-coupled production. You can also see maximal gene deletions.

Model : iPC815 [2].
Target metabolite : nadph_c
List of minimal gene deletion strategies (Download)

Gene deletion strategy (21 of 24: See next) for growth-coupled production (at least stoichioemetrically feasible)
  Gene deletion size : 11
  Gene deletion: YPO2066 YPO0918 YPO2161 YPO0254 YPO2288 YPO1109 YPO3142 YPO3358 YPO0914 YPO1133 YPO0439   (List of alternative genes)
  Computed by: RandTrimGdel [1] (Step 1, Step 2)

When growth rate is maximized,
  Growth Rate : 0.255599 (mmol/gDw/h)
  Minimum Production Rate : 0.043796 (mmol/gDw/h)

Substrate: (mmol/gDw/h)
  EX_h_e : 7.839389
  EX_cit_e : 7.400000
  EX_o2_e : 5.233640
  EX_gly_e : 2.200000
  EX_cys__L_e : 1.000000
  EX_glc__D_e : 0.372461
  EX_pi_e : 0.340655
  EX_lys__L_e : 0.063900
  EX_phe__L_e : 0.048564
  EX_met__L_e : 0.035784
  EX_tyr__L_e : 0.033228
  EX_pnto__R_e : 0.000015

Product: (mmol/gDw/h)
  EX_co2_e : 14.187573
  EX_h2o_e : 8.315523
  EX_succ_e : 7.082545
  EX_h2s_e : 0.981326
  EX_ac_e : 0.410000
  Auxiliary production reaction : 0.043796
  EX_ade_e : 0.001789

Visualization
  1. Download JSON file.
  2. Go to Escher site [3].

References
[1] Tamura, T. MetNetComp: Database for minimal and maximal gene deletion strategies for growth-coupled production of genome-scale metabolic networks, IEEE/ACM Transactions on Computational Biology and Bioinformatics, in press.
[2] Norsigian, C. J., Pusarla, N., McConn, J. L., Yurkovich, J. T., Dräger, A., Palsson, B. O., & King, Z. (2020). BiGG Models 2020: multi-strain genome-scale models and expansion across the phylogenetic tree. Nucleic acids research, 48(D1), D402-D406.
[3] King, Z. A., Dräger, A., Ebrahim, A., Sonnenschein, N., Lewis, N. E., & Palsson, B. O. (2015). Escher: a web application for building, sharing, and embedding data-rich visualizations of biological pathways. PLoS computational biology, 11(8), e1004321.

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Last updated: 27-Sep-2023
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